We used nanoscale flow cytometry to enumerate PMPs which were defined as submicron events (100-1000nm) immunoreactive to anti-PSMA mAb when compared to isotype control labeled samples. Patients and Methods: Plasmas from healthy volunteers, benign prostatic hyperplasia patients, and PCa patients with various Gleason score patterns were analyzed for PMPs. We hypothesize that enumeration of circulating prostate microparticles (PMPs), a type of extracellular vesicle (EV), can identify patients with Gleason Score≥4+4 prostate cancer (PCa) in a manner independent of PSA. Received: DecemAccepted: JanuPublished: JanuAbstractīackground: Extracellular vesicles released by prostate cancer present in seminal fluid, urine, and blood may represent a non-invasive means to identify and prioritize patients with intermediate risk and high risk of prostate cancer. Keywords: prostate microparticles, extracellular vesicles, nanoscale flow cytometry, prostate cancer, liquid biopsy * These authors have contributed equally to this work Leong 1,3ġ Department of Surgery, Western University, London, CanadaĢ Department of Oncology, Western University, London, Canadaģ Translational Prostate Cancer Research Laboratory, Lawson Health Research Institute, London, CanadaĤ University Health Network, University of Toronto, Toronto, Canadaĥ Sandia National Laboratories, Albuquerque, NM, USAĦ Department of Urology, University Medical Center Freiburg, Freiburg, Germanyħ Department of Urology, University of Dammam, Dammam, Saudi ArabiaĨ Department of Mechanical and Materials Engineering, Western University, London, Canada

Guo 8, Jun Yang 8, Philipp Wolf 6, Nicholas E. Al-Zahrani 1,3,7, Siddika Pardhan 1,3, Sabine I.

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